Cryopreservation

Introduction

Cryopreservation is a process where cells or whole tissues are preserved.

Frozen tissue can be stored indefinitely in liquid nitrogen at -196°C. The freezing of human sperm is an old and well recognised procedure.


Sperm Freezing/Banking

Both husband or donor sperm can be freezed for IVF or IUI at a later date, but sperm banking provides backup option if a sample cannot be collected on demand or if sample quality is poor on the day when the eggs are available.


Oocyte Banking

The cryopreservation of human oocytes/eggs would be advantageous to the patient with ovarian disease or any other condition that limits egg production.


Embryo Freezing

Embryo cryopreservation has been employed successfully for nearly 20 years. Embryo freezing is used to provide alternatives for the ART couple for whom the risk of multiple pregnancy restricts the number of embryos that can be transferred during the treatment cycle.Additionally, embryo banking is done when embryos cannot be transferred during the egg pickup cycle.


How is the Semen obtained in Semen Banking?

A donor who donates the semen after he is screened and tested for various infections such as HIV, Hepatities and other Venereal Disease.


What is Artificial Insemination with Donor sample

sample (AID) or Donor Insemination (DI): In as many as 30% of infertile couples, the male is responsible for infertility. A significant percentage of these males do not have children despite various treatments including ICSI. The solution is AID or DI.


Who can benefit from AID?

AID (Artificial Insemination Dnr) is generally suggested for males with low sperm counts ( Oligozoospermia) or zero counts ( Azoospermia), where treatment with drugs, surgery and ART like AIH, IUI and ICSI have proven unsuccessful. It is an acceptable alternative to adoption.



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