IVF (In Vitro Fertilization)
In Vitro Fertilisation : In Vitro Fertilisation treatment, the sperm has to unite with the egg, which is released from the ovary in order to achieve pregnancy. Normally this union is called fertilization, it occurs with in the fallopian tube. However, in IVF the union occurs in the laboratory in which egg and sperm have been collected and cultured together to form embryos and then embryos are transferred to the uterus on day 3 to continue growth. This treatment is called In Vitro Fertilisation treatment and this treatment is done successfully in the best fertility centre in Hyderabad, Motherhood fertility centre.
Candidate selection for IVF
Both fallopian tubes are absent or blocked due to surgery or Tubal pregnancy or infection (STD or Tuberculosis) or Endometriosis
Patients with unexplained infertility where all the investigations reports normal, but who still do not conceive with routine treatments.
Patients who have failed to become pregnant in spite of all routine treatments of infertility.
The growth and development of the eggs is closely monitored by repeated ultrasound studies and ovulation itself is triggered by an injection (Human Chorionic Gonadotropin), after which the egg retrieval is scheduled.
Procedure: Five major steps in the In Vitro Fertilization treatment and embryo transfer sequence:
Monitor the development of ripening egg(s) in the ovaries.
Collection of eggs.
Obtaining the sperm.
Putting the eggs and sperm together in the laboratory and providing optional conditions for fertilization and early embryo growth.
Transferring the embryos into the uterus.
(Collection of Eggs): Eggs are gently removed from ovaries using the needle. This is called “follicular aspiration. “They are placed with the sperm in incubators to allow fertilization to take place.
Obtaining the sperms: The semen from man is obtained by masturbation in a sterile container and handed over to embryologist for the processing of semen, for separation of sperms from the semen plasma, other debris and immotile cells. The processed active motile sperms are selected and used for In Vitro Fertilization treatment /ICSI fertility treatment (Intracytoplasmic Sperm Injection) procedures. For patients /couples having recurrent failures in implantation and miscarriages it is mandatory to undergo DNA fragmentation test before undergoing In Vitro Fertilization treatment /ICSI treatment procedures.
DNA Fragmentation Test: We can assess cell death by detecting DNA fragments, using agarose gel electrophoresis. One of the classic features of apoptosis is the cleavage of the genomic DNA into oligonucleosomal fragments. It is represented by multiples of 180-200bp.
This test may help to identify men who are at risk of failing to initiate a pregnancy. Information about sperm DNA integrity may help in the clinical diagnosis, management and treatment of male infertility and may be of prognostic value in assessing outcome of assisted conception treatment.
Fertilisation: The eggs are placed with the sperm in incubators to allow fertilization to take place. Normally in In Vitro Fertilization treatment one egg is mixed with 100,000 sperms and one of the sperms fertilizes the egg on its own. The eggs are examined carefully at intervals to ensure that fertilization and cell division have taken place. The fertilized eggs are now called embryos. Embryos are usually placed in the women’s uterus 2 or 3 days after egg retrieval. The patient is informed to consult with the best infertility specialist in Hyderabad in Motherhood fertility centre for Serum BHCG test after 14 days of medication or, can do it in any lab nearby and can inform the obtained result value to confirm the pregnancy.
In vitro fertilization treatment is the most widely known assisted reproductive technology (ART), which is utilized to help the couple to have a baby. In this In Vitro Fertilization hormone treatment procedure, the fertilization process is done outside of the body in the laboratory. Then an embryo is generated in the lab and then the fertilized egg is placed inside the womb of the woman or to the surrogate mother, who is carrying the child and being pregnant for the couple in this In Vitro Fertilization treatment. When the quality as well as the quantity of sperm is low, this process is done in the IVF fertility centres. You can choose Motherhood fertility centre, one of the best Fertility Treatment Centers in Hyderabad for this In Vitro Fertilization treatment.
You can also generate a better understanding of common laboratory procedures used during an In Vitro Fertilization treatment cycle:
Egg Retrieval: it is a small surgical process in which the infertility specialist obtains eggs from the follicles of the ovaries of the mother.
Donor Insemination: A specialist places the sperm directly inside the vagina of the mother at the cervix or inside the uterus, which is called Intrauterine Insemination or IUI fertility treatment process. Before this process donor was called Artificial Insemination.
Embryo Development: When the sperm fertilizes the egg, an embryo is created and starts to grow. When the embryo is of 2 to 3 days old, at the ‘cleavage stage’ at 5 to 6 days, it is called as a ‘blastocyst procedure’.
Intra-Cytoplasmic Sperm Injection (ICSI): in this process only one sperm is injected into an egg to stimulate fertilization. This ICSI treatment procedure is done with special equipment in the embryology laboratory.
Assisted Hatching: A laser or chemical solution is used on the embryo to increase the chances of implantation in the lining of the uterus, called as endometrium.
Embryo Transfer: Fertilized eggs or embryos are placed back into the uterus of the mother or fallopian tube through the cervix.
Embryo Cryopreservation: At extreme cold temperatures, an embryo becomes frozen. The preserved embryos can be preserved for the future.
In Vitro Fertilization hormone treatment includes many steps and it takes several months to complete. Sometimes it will be successful on the first attempt but most of the couple require more than 1 round of IVF to get pregnant. This IVF technique definitely enhances your chances of getting pregnant. Believe that God is preparing something great for you, just hold on. Maybe we, Motherhood fertility centre, the best fertility hospital in Hyderabad is here with you by following the instructions of God.